(A) Schematic of the Scaffold map algorithm. This foundational reference map provides a working definition of systemic immune organization to which new data can be integrated to reveal deviations driven by genetics, environment, or pathology.Ĭopyright © 2015, American Association for the Advancement of Science. The approach revealed influences of genetic variation and circadian rhythms on immune system structure, enabled direct comparisons of murine and human blood cell phenotypes, and even enabled archival fluorescence-based flow cytometry data to be mapped onto the reference framework.
The maps recapitulated canonical cellular phenotypes and revealed reproducible, tissue-specific deviations. We developed graphical approaches to construct an extensible immune reference map from mass cytometry data of cells from different organs, incorporating landmark cell populations as flags on the map to compare cells from distinct samples. cells function in an interacting hierarchy that coordinates the activities of various cell types according to genetic and environmental contexts. Program in Immunology, Stanford University, Stanford, CA 94305, USA. 6 Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.5 Department of Obstetrics and Gynecology, Division of Gynecologic Oncology, Stanford University, Stanford, CA 94305, USA.4 Department of Pathology, Stanford University, Stanford, CA 94305, USA.3 Department of Pathology, Stanford University, Stanford, CA 94305, USA.2 Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA. Department of Pathology, Stanford University, Stanford, CA 94305, USA. 1 Baxter Laboratory in Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.
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